![]() We then designed a small-molecule inhibitor that efficiently blocks HtrA activity, E-cadherin cleavage and intercellular entry of H. pylori designed to access the intercellular space. E-cadherin shedding disrupts epithelial barrier functions allowing H. an EVOM Voltohmmeter (EVOL, World Precision Instruments, Aston, Herts, UK) with a STX-2 chopstick electrodes (WPI, Sarasota, Florida, USA) (Inoue et al. Here, we describe an entirely new function of HtrA and identify it as a new secreted virulence factor from Helicobacter pylori, which cleaves the ectodomain of the cell-adhesion protein E-cadherin. Mammalian and prokaryotic high-temperature requirement A (HtrA) proteins are chaperones and serine proteases with important roles in protein quality control. The Millicell-ERS (Electrical Resistance System) reliably measures membrane potential & resistance of epithelial cells in culture. Measurement of Transepithelial Electrical Resistance (TEER), Cell Monolayer Health Assessment, Quantitative Measurement of Confluence Epithelial cells grown alone or in coculture with stromal cells were treated with Oe (2). ![]() Once the Millicell® ERS-2 system is standardized, you can use it to quantitatively measure cell confluence. Transepithelial resistance (TER) was monitored with an EVOM voltohmmeter to determine monolayer polarity and integrity. Membrane capacitance does not affect resistance readings. Widely cited and recommended in epithelial cell culture protocols Measures trans-epithelial electrical resistance or trans-epithelilal voltage Compatible with 12 and 24 well culture plate systems out of the box Includes. There is no electrochemical deposition of electrode metals. Measurements do not compromise sterility or damage cells on the membrane Portable instrument that fits into laminar flow hoods Accessories include a replacement (fixed) electrode set, adjustable electrode set and a specialized electrode set for use with Millicell®-96 cell culture insert plates. Due to the small size of the electrodes, you can easily measure transepithelial voltage and the resistance of cells grown on microporous membranes. A silver/silver chloride (Ag/AgCl) pellet on each electrode tip measures voltage. This device qualitatively measures cell monolayer health and quantitatively measures cell confluence. The Millicell® ERS-2 (Electrical Resistance System) reliably measures membrane potential and resistance of epithelial cells in culture. Using a EVOM voltohmmeter from World Precision Instruments, sterilize the STX2 electrode probe (chopsticks) in ethanol for no more than 5 minutes.
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